Molecular weight (DA)
37kD
Immunogen
The antiserum was produced against synthesized peptide derived from human RAD51 around the phosphorylation site of Tyr315. AA range:281-330
Specificity
Phospho-Rad51 (Y315) Polyclonal Antibody detects endogenous levels of Rad51 protein only when phosphorylated at Y315.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009],
Function
disease:Defects in RAD51 are associated with breast cancer (BC) [MIM:114480].,function:May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.,PTM:Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.,similarity:Belongs to the recA family.,similarity:Belongs to the recA family. RAD51 subfamily.,similarity:Contains 1 HhH domain.,subcellular location:Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage.,subunit:Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L an
Protein name
DNA repair protein RAD51 homolog 1
Other name
RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
Fields
>>Homologous recombination;>>Fanconi anemia pathway;>>Pathways in cancer;>>Pancreatic cancer
Human protein sequence Database
Q06609
Mouse protein sequence database
Q08297
Rat protein sequence database
Cellular localization
Nucleus . Cytoplasm . Cytoplasm, perinuclear region. Mitochondrion matrix . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage (PubMed:20154705). DNA damage induces an increase in nuclear levels (PubMed:20154705). Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment (PubMed:23754376). Accumulated at sites of DNA damage in a SPIDR-dependent manner (PubMed:23509288). Recruited at sites of DNA damage in a MCM9-MCM8-dependent manner (PubMed:23401855). Colocalizes with ERCC5/XPG to nuclear foci in S phase (PubMed:26833090). .
Tissue expression
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
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