Molecular weight (DA)
26kD
Immunogen
The antiserum was produced against synthesized peptide derived from human BCL2 around the phosphorylation site of Ser70. AA range:36-85
Specificity
Phospho-Bcl-2 (S70) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at S70.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
BCL2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Protein name
Apoptosis regulator Bcl-2
Other name
BCL2; Apoptosis regulator Bcl-2
Human protein sequence Database
Mouse protein sequence database
Rat protein sequence database
P49950