Molecular weight (DA)
28kD
Immunogen
The antiserum was produced against synthesized peptide derived from human HINT1. AA range:71-120
Specificity
Hint1 Polyclonal Antibody detects endogenous levels of Hint1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015],
Function
caution:Was originally thought to be a protein kinase C inhibitor and to bind zinc in solution. Both seem to be incorrect.,domain:The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.,function:Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.,similarity:Belongs to the HINT family.,similarity:Contains 1 HIT domain.,subcellular location:Interaction with CDK7 leads to a more nuclear localization.,subunit:Homodimer. Interacts with CDK7.,tissue specificity:Widely expressed.,
Protein name
Histidine triad nucleotide-binding protein 1
Other name
HINT1; HINT; PKCI1; PRKCNH1; Histidine triad nucleotide-binding protein 1; Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1
Human protein sequence Database
P49773
Mouse protein sequence database
P70349
Rat protein sequence database
P62959
Cellular localization
Cytoplasm . Nucleus . Interaction with CDK7 leads to a more nuclear localization. .
Tissue expression
Widely expressed.
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