Molecular weight (DA)
22kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human BNIP3. AA range:71-120
Specificity
BNIP-3 Polyclonal Antibody detects endogenous levels of BNIP-3 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014],
Function
function:Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.,similarity:Belongs to the NIP3 family.,subcellular location:Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope.,subunit:Homodimer. Binds to BCL2. Interacts with BNIP3L. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1.,
Protein name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Other name
BNIP3; NIP3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Fields
>>FoxO signaling pathway;>>Mitophagy - animal;>>Autophagy - animal;>>Shigellosis;>>Legionellosis
Human protein sequence Database
Q12983
Mouse protein sequence database
O55003
Rat protein sequence database
Cellular localization
Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.
Tissue expression
B-cell,Brain,Cerebellum,Kidney,Testis,
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