Immunogen
Purified recombinant fragment of ApoA-IV (aa21-396) expressed in E. Coli.
Specificity
ApoA-IV Monoclonal Antibody detects endogenous levels of ApoA-IV protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008],
Function
domain:Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.,function:May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.,online information:The Singapore human mutation and polymorphism database,polymorphism:Eight alleles have been characterized (APOA-IV*0 to APOA-IV*7). APOA-IV*1 is the major allele (90%), APOA-IV*2 is also common (8%), the others are rare alleles.,similarity:Belongs to the apolipoprotein A1/A4/E family.,tissue
Protein name
Apolipoprotein A-IV
Other name
APOA4; Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4
Fields
>>Fat digestion and absorption;>>Vitamin digestion and absorption;>>Cholesterol metabolism;>>Lipid and atherosclerosis
Human protein sequence Database
P06727
Mouse protein sequence database
P06728
Rat protein sequence database
Cellular localization
Secreted.
Tissue expression
Synthesized primarily in the intestine and secreted in plasma.
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