Molecular weight (DA)
86kD
Immunogen
The antiserum was produced against synthesized peptide derived from human HRS around the phosphorylation site of Tyr334. AA range:301-350
Specificity
Phospho-Hrs (Y334) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y334.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010],
Function
domain:Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.,function:Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the
Protein name
Hepatocyte growth factor-regulated tyrosine kinase substrate
Other name
HGS; HRS; Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110
Fields
>>Viral life cycle - HIV-1;>>Endocytosis;>>Phagosome
Human protein sequence Database
O14964
Mouse protein sequence database
Q99LI8
Rat protein sequence database
Q9JJ50
Cellular localization
Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome, multivesicular body membrane ; Peripheral membrane protein . Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments. .
Tissue expression
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
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