Immunogen
Purified recombinant fragment of human GATA-5 expressed in E. Coli.
Specificity
GATA-5 Monoclonal Antibody detects endogenous levels of GATA-5 protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. [provided by RefSeq, Jul 2008],
Function
function:Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer. May play an important role in the transcriptional program(s) that underlies smooth muscle cell diversity.,similarity:Contains 2 GATA-type zinc fingers.,
Protein name
Transcription factor GATA-5
Other name
GATA5; Transcription factor GATA-5; GATA-binding factor 5
Human protein sequence Database
Q9BWX5
Mouse protein sequence database
P97489
Rat protein sequence database
Cellular localization
Nucleus .
Tissue expression
Uterus,
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