Immunogen
Purified recombinant fragment of human Gfi-1 expressed in E. Coli.
Specificity
Gfi-1 Monoclonal Antibody detects endogenous levels of Gfi-1 protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008],
Function
disease:Defects in GFI1 are a cause of autosomal dominant severe congenital neutropenia (SCN) [MIM:202700].,disease:Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.,domain:The Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.,function:May be a transcription factor involved in regulating the expression of genes active in the S phase during cell cycle progression in T-cells. May be involved in tumor progression (By similarity). Represses ELA2 transcription.,online information:GFI1 mutation db,similarity:Contains 6 C2H2-type zinc fingers.,subunit:Interacts with U2AF1L4.,
Protein name
Zinc finger protein Gfi-1
Other name
GFI1; ZNF163; Zinc finger protein Gfi-1; Growth factor independent protein 1; Zinc finger protein 163
Human protein sequence Database
Q99684
Mouse protein sequence database
P70338
Rat protein sequence database
Cellular localization
Nucleus . Colocalizes with PIAS3 and RUNX1T1 in nuclear dots.
Tissue expression
Bone marrow,Lung,Spleen,Testis,Thymus,
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