Immunogen
Purified recombinant human TFIIB protein fragments expressed in E.coli.
Specificity
TFIIB Monoclonal Antibody detects endogenous levels of TFIIB protein.
Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Immunohistochemistry: 1/500 - 1/1000. Immunofluorescence: 1/100 - 1/500. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
general transcription factor IIB(GTF2B) Homo sapiens This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008],
Function
cofactor:Binds 1 zinc ion per subunit.,function:General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.,similarity:Belongs to the TFIIB family.,similarity:Contains 1 TFIIB-type zinc finger.,subunit:Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 (By similarity). Interacts with Epstein-Barr virus EBNA2.,
Protein name
Transcription initiation factor IIB
Other name
GTF2B; TF2B; TFIIB; Transcription initiation factor IIB; General transcription factor TFIIB; S300-II
Fields
>>Basal transcription factors;>>Spinocerebellar ataxia;>>Viral carcinogenesis
Human protein sequence Database
Q00403
Mouse protein sequence database
P62915
Rat protein sequence database
P62916
Cellular localization
Nucleus . Chromosome . Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171). .
Tissue expression
Expressed in the inner cell mass forming the embryoblast (PubMed:24441171). Not detected in cells from the outer thin layer trophoblast (at protein level) (PubMed:24441171).
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