Molecular weight (DA)
60kD
Immunogen
Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
Specificity
This antibody detects endogenous levels of SMAD5 (C-terminus) and does not cross-react with related proteins.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
wb 1:1000 icc 1:75 fcm 1:100
Purification process (Immunogen)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
Function
function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.,tissue specificity:Ubiquitous.,
Other name
DKFZp781C1895;DKFZp781O1323;Dwfc;hSmad 5;hSmad5;JV5 1;JV5-1;MAD homolog 5;MAD mothers against decapentaplegic homolog 5;MAD, mothers against decapentaplegic homolog 5;MADH 5;MADH5;Mothers against decapentaplegic homolog 5;Mothers against DPP homolog 5;MusMLP; SMA and MAD related protein 5;SMAD 5;SMAD family member 5;SMAD mothers against DPP homolog 5;Smad5;Smad5;SMAD5_HUMAN.
Fields
>>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
Human protein sequence Database
Q99717
Mouse protein sequence database
P97454
Rat protein sequence database
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Tissue expression
Ubiquitous.
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