Immunogen
The antiserum was produced against synthesized peptide derived from human APLF around the phosphorylation site of Ser116. AA range:82-131
Specificity
Phospho-APLF (S116) Polyclonal Antibody detects endogenous levels of APLF protein only when phosphorylated at S116.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM, Mar 2008],
Function
domain:The FHA-like domain mediates interaction with XRCC1 and XRCC4.,function:Involved in single-strand and double-strand DNA break repair.,PTM:Phosphorylated in an ATM-dependent manner upon double-strand DNA break.,similarity:Contains 1 FHA-like domain.,similarity:Contains 2 C2H2-type zinc fingers.,subcellular location:Colocalizes with XRCC1 at sites of DNA damage.,subunit:Interacts with XRCC1. May also interact with XRCC4 and XRCC5.,
Protein name
Aprataxin and PNK-like factor
Other name
APLF; C2orf13; PALF; XIP1; Aprataxin and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; XRCC1-interacting protein 1
Human protein sequence Database
Q8IW19
Mouse protein sequence database
Q9D842
Rat protein sequence database
Cellular localization
Nucleus . Chromosome . Cytoplasm, cytosol . Localizes to DNA damage sites (PubMed:18474613, PubMed:18172500, PubMed:21211721, PubMed:23689425). Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers (PubMed:18172500). .
Tissue expression
Testis,
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