Molecular weight (DA)
50kD
Immunogen
The antiserum was produced against synthesized peptide derived from human RNF14. AA range:361-410
Specificity
ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011],
Function
caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,domain:The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.,domain:The RING-type zinc finger is essential for the interaction with UBE2E2.,function:Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.,pathway:Protein modification; protein ubiquitination.,PTM:RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.,similarity:Be
Protein name
E3 ubiquitin-protein ligase RNF14
Other name
RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein
Human protein sequence Database
Q9UBS8
Mouse protein sequence database
Q9JI90
Rat protein sequence database
Cellular localization
Cytoplasm . Nucleus .
Tissue expression
Widely expressed.
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