Welcome to Nanjing Byabscience    HOTLINE: 800-880-8748 |
Location: Home > Products > Primary Antibodies > Polyclonal antibody

Smad2/3 Polyclonal Antibody

Polyclonal antibody

Specification

BYab-02016

  • 50UL $180 100UL $255
  • Delivery: In Stock

Product introduction Experimental scheme Citation Related products

Product introduction>

Host
Reactiveness
Use
Molecular weight (DA)
48kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad2/3. AA range:1-50
Specificity
Smad2/3 Polyclonal Antibody detects endogenous levels of Smad2/3 protein.
Source
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation
Function
disease:Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.,function:Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.,PTM:Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo.,PTM:In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.,PTM:Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degr
Gene Name
SMAD2/SMAD3
Protein name
Mothers against decapentaplegic homolog 2/3
Abbreviation
Smad2/3
Other name
SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD3; MADH3; Mothers against decapentaplegic
Fields
>>Cell cycle;>>Endocytosis;>>Cellular senescence;>>TGF-beta signaling pathway;>>Apelin signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Th17 cell differentiation;>>Relaxin signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Chagas disease;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Colorectal cancer;>>Pancreatic cancer;>>Hepatocellular carcinoma;>>Gastric cancer;>>Inflammatory bowel disease;>>Diabetic cardiomyopathy
Human gene ID
4087/4088
Human protein sequence Database
Q15796/P84022
Mouse gene ID
17126/17127
Mouse protein sequence database
Rat gene ID
29357/25631
Rat protein sequence database
O70436/P84025
Cellular localization
Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). .
Tissue expression
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Storage
-20°C/1 year

Experimental scheme>

Procedure

Citation(0)>

成功添加到购物车

查看购物车 继续购物

Smad2/3 Polyclonal Antibody

亮暗模式切换
X

Online
Service

Online Service
08:30 - 17:30

service hotline

Service
Hotline

Customer service hotline

800-880-8748
Customer service hotline

扫码

Scan
Wechat

微信二维码 Scan wechat
返回顶部 TOP