Molecular weight (DA)
53kD
Immunogen
The antiserum was produced against synthesized peptide derived from human TAF1A. AA range:301-350
Specificity
TAF I p48 Polyclonal Antibody detects endogenous levels of TAF I p48 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq, Jan 2011],
Function
function:Component of the transcription factor SL1/TIFIB complex involved in the assembly of the PIC (preinitiation complex).,subunit:Component of the transcription factor SL1/TIFIB complex which is composed of TBP and at least TAF1A, TAF1B and TAF1C. In the complex, interacts directly with TBP, TAF1B and TAF1C. Interaction of the SL1/TIFIB subunits with TBP excludes interaction with the transcription factor IID (TFIID) subunits. This mutually exclusive binding directs the formation of promoter and RNA polymerase-selective TBP-TAF complexes.,
Protein name
TATA box-binding protein-associated factor RNA polymerase I subunit A
Other name
TAF1A; TATA box-binding protein-associated factor RNA polymerase I subunit A; RNA polymerase I-specific TBP-associated factor 48 kDa; TAFI48; TATA box-binding protein-associated factor 1A; TBP-associated factor 1A; Transcription factor SL1;
Human protein sequence Database
Q15573
Mouse protein sequence database
P97357
Rat protein sequence database
Q3B7U2
Cellular localization
Nucleus.
Tissue expression
Embryo,Skin,
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