Molecular weight (DA)
80kD
Immunogen
The antiserum was produced against synthesized peptide derived from human GCF. AA range:141-190
Specificity
TCF-9 Polyclonal Antibody detects endogenous levels of TCF-9 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene. [provided by RefSeq, Jul 2008],
Function
function:Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.,sequence caution:Contaminating sequence. The N-terminus matches the 2q37.3 region.,similarity:Belongs to the GCF family.,tissue specificity:Widely expressed in tissues and cell lines.,
Protein name
GC-rich sequence DNA-binding factor 2
Other name
GCFC2; C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9
Human protein sequence Database
P16383
Mouse protein sequence database
Q8BKT3
Rat protein sequence database
Cellular localization
Nucleus, nucleoplasm . Nucleus, nucleolus .
Tissue expression
Widely expressed in tissues and cell lines.
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