Molecular weight (DA)
33kD
Immunogen
The antiserum was produced against synthesized peptide derived from human TF2E2. AA range:151-200
Specificity
TFIIE-β Polyclonal Antibody detects endogenous levels of TFIIE-β protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
function:Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.,similarity:Belongs to the TFIIE beta subunit family.,similarity:Contains 1 TFIIE beta DNA-binding domain.,subunit:Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H.,
Function
function:Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.,similarity:Belongs to the TFIIE beta subunit family.,similarity:Contains 1 TFIIE beta DNA-binding domain.,subunit:Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H.,
Protein name
Transcription initiation factor IIE subunit beta
Other name
GTF2E2; TF2E2; Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2
Fields
>>Basal transcription factors;>>Viral carcinogenesis
Human protein sequence Database
P29084
Mouse protein sequence database
Q9D902
Rat protein sequence database
Cellular localization
Nucleus .
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