Molecular weight (DA)
265kD
Immunogen
The antiserum was produced against synthesized peptide derived from human ACC1 around the phosphorylation site of Ser80. AA range:46-95
Specificity
Phospho-ACCα (S80) Polyclonal Antibody detects endogenous levels of ACCα protein only when phosphorylated at S80.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA.,catalytic activity:ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.,cofactor:Binds 2 manganese ions per subunit.,cofactor:Biotin.,disease:Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth.,enzyme regulation:By phosphorylation.,function:Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.,online information:Acetyl-CoA carboxylase entry,pathway:Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: st
Protein name
Acetyl-CoA carboxylase 1
Other name
ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha
Fields
>>Fatty acid biosynthesis;>>Pyruvate metabolism;>>Propanoate metabolism;>>Metabolic pathways;>>Fatty acid metabolism;>>AMPK signaling pathway;>>Insulin signaling pathway;>>Glucagon signaling pathway;>>Alcoholic liver disease
Human protein sequence Database
Q13085
Mouse protein sequence database
Q5SWU9
Rat protein sequence database
P11497
Cellular localization
Cytoplasm, cytosol .
Tissue expression
Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
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