Molecular weight (DA)
58kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 1A1/2. AA range:71-120
Specificity
CYP1A1/2 Polyclonal Antibody detects endogenous levels of CYP1A1/2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016],
Function
catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,cofactor:Heme group.,function:Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.,induction:By 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).,online information:CYP1A1 alleles,online information:CYP1A1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Lung, lymphocytes and placenta.,
Protein name
Cytochrome P450 1A1/2
Other name
CYP1A1; Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A2; Cytochrome P450 1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450-P3
Fields
>>Steroid hormone biosynthesis;>>Tryptophan metabolism;>>Retinol metabolism;>>Metabolism of xenobiotics by cytochrome P450;>>Metabolic pathways;>>Ovarian steroidogenesis;>>Chemical carcinogenesis - DNA adducts;>>Chemical carcinogenesis - receptor activation;>>Chemical carcinogenesis - reactive oxygen species;>>Lipid and atherosclerosis
Human protein sequence Database
P04798/P05177
Mouse gene ID
13076/13077
Mouse protein sequence database
Rat protein sequence database
P00185/P04799
Cellular localization
Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion inner membrane ; Peripheral membrane protein . Microsome membrane ; Peripheral membrane protein . Cytoplasm .
Tissue expression
Lung, lymphocytes and placenta.
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车