Molecular weight (DA)
38kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 24A1. AA range:411-460
Specificity
CYP24A1 Polyclonal Antibody detects endogenous levels of CYP24A1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
cytochrome P450 family 24 subfamily A member 1(CYP24A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:Calcidiol + NADPH + O(2) = secalciferol + NADP(+) + H(2)O.,catalytic activity:Calcitriol + NADPH + O(2) = calcitetrol + NADP(+) + H(2)O.,cofactor:Heme group.,function:Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.,similarity:Belongs to the cytochrome P450 family.,
Protein name
1,25-dihydroxyvitamin D(3) 24-hydroxylase mitochondrial
Other name
CYP24A1; CYP24; 1; 25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; Vitamin D(3) 24-hydroxylase; Cytochrome P450 24A1; Cytochrome P450-CC24
Fields
>>Steroid biosynthesis;>>Metabolic pathways;>>Parathyroid hormone synthesis, secretion and action;>>MicroRNAs in cancer
Human protein sequence Database
Q07973
Mouse protein sequence database
Q64441
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
PCR rescued clones,
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