Immunogen
The antiserum was produced against synthesized peptide derived from human HIBADH. AA range:281-330
Specificity
HIBADH Polyclonal Antibody detects endogenous levels of HIBADH protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011],
Function
catalytic activity:3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH.,similarity:Belongs to the 3-hydroxyisobutyrate dehydrogenase family.,subunit:Homodimer.,
Protein name
3-hydroxyisobutyrate dehydrogenase mitochondrial
Other name
HIBADH; 3-hydroxyisobutyrate dehydrogenase; mitochondrial; HIBADH
Fields
>>Valine, leucine and isoleucine degradation;>>Metabolic pathways
Human protein sequence Database
P31937
Mouse protein sequence database
Q99L13
Rat protein sequence database
P29266
Cellular localization
Mitochondrion.
Tissue expression
Detected in skin fibroblasts.
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