Molecular weight (DA)
70kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP-16. AA range:551-600
Specificity
MMP-16 Polyclonal Antibody detects endogenous levels of MMP-16 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010],
Function
cofactor:Binds 1 zinc ion per subunit.,cofactor:Calcium.,developmental stage:Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:TIMP-2 shows little inhibitory activity compared to TIMP-1. TIMP-1 seems to have less binding affinity than TIMP-2 for the short isoform.,function:Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. The short isoform cleaves fibronectin and also collagen type III, but at low
Protein name
Matrix metalloproteinase-16
Other name
MMP16; MMPX2; Matrix metalloproteinase-16; MMP-16; MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP
Fields
>>Parathyroid hormone synthesis, secretion and action;>>MicroRNAs in cancer
Human protein sequence Database
P51512
Mouse protein sequence database
Q9WTR0
Rat protein sequence database
O35548
Cellular localization
[Isoform Long]: Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Localized at the cell surface of melanoma cells.; [Isoform Short]: Secreted, extracellular space, extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Tissue expression
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
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