Molecular weight (DA)
150kD
Immunogen
The antiserum was produced against synthesized peptide derived from human PLCG1. AA range:736-785
Specificity
PLC γ1 Polyclonal Antibody detects endogenous levels of PLC γ1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.,cofactor:Calcium.,domain:The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.,function:PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.,PTM:The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.,PTM:Ubiquitinated by CBLB in activated T-cells.,similarity:Contains 1 C2 domain.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 PI-PLC X-box domain.,similarity:Contains 1 PI-PLC Y-box domain.,similarity:Contains 1 SH3 domain.,simil
Protein name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Other name
PLCG1; PLC1; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1
Fields
>>Inositol phosphate metabolism;>>Metabolic pathways;>>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Neutrophil extracellular trap formation;>>Natural killer cell mediated cytotoxicity;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Neurotrophin signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>Thyroid hormone signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Growth hormone synthesis, secretion and action;>>Parkinson disease;>>Pathways of neurodegeneration - multiple diseases;>>Vibrio
Human protein sequence Database
P19174
Mouse protein sequence database
Q62077
Rat protein sequence database
P10686
Cellular localization
Cell projection, lamellipodium . Cell projection, ruffle . Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. .
Tissue expression
Brain,Epithelium,Testis,Vein,
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