Molecular weight (DA)
38kD
Immunogen
The antiserum was produced against synthesized peptide derived from human AOS1. AA range:221-270
Specificity
SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
SUMO1 activating enzyme subunit 1(SAE1) Homo sapiens Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010],
Function
function:The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.,pathway:Protein modification; protein sumoylation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2.,tissue specificity:Expression level increases during S phase and drops in G2 phase (at protein level).,
Protein name
SUMO-activating enzyme subunit 1
Other name
SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A
Fields
>>Ubiquitin mediated proteolysis
Human protein sequence Database
Q9UBE0
Mouse protein sequence database
Q9R1T2
Rat protein sequence database
Q6AXQ0
Cellular localization
Nucleus .
Tissue expression
Expression level increases during S phase and drops in G2 phase (at protein level).
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