Molecular weight (DA)
70kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CYBB. AA range:111-160
Specificity
gp91-phox Polyclonal Antibody detects endogenous levels of gp91-phox protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008],
Function
cofactor:FAD.,disease:Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.,online information:CYBB deficiency database,PTM:Glycosylated.,similarity:Contains 1 FAD-binding FR-t
Protein name
Cytochrome b-245 heavy chain
Other name
CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome
Fields
>>HIF-1 signaling pathway;>>Phagosome;>>Ferroptosis;>>Necroptosis;>>Neutrophil extracellular trap formation;>>NOD-like receptor signaling pathway;>>Leukocyte transendothelial migration;>>AGE-RAGE signaling pathway in diabetic complications;>>Alzheimer disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Leishmaniasis;>>Coronavirus disease - COVID-19;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis
Human protein sequence Database
P04839
Mouse protein sequence database
Q61093
Rat protein sequence database
Cellular localization
Cell membrane; Multi-pass membrane protein. As unassembled monomer may localize to the endoplasmic reticulum. .
Tissue expression
Detected in neutrophils (at protein level).
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