Molecular weight (DA)
43kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human BCAT1. AA range:231-280
Specificity
ECA39 Polyclonal Antibody detects endogenous levels of ECA39 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
branched chain amino acid transaminase 1(BCAT1) Homo sapiens This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010],
Function
catalytic activity:2-oxoglutaric acid + L-isoleucine = (S)-3-methyl-2-oxopentanoic acid + L-glutamic acid.,catalytic activity:2-oxoglutaric acid + L-valine = 3-methyl-2-oxobutanoic acid + L-glutamic acid.,catalytic activity:L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.,cofactor:Pyridoxal phosphate.,function:Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.,similarity:Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer.,tissue specificity:During embryogenesis, expressed in the brain and kidney. Overexpressed in C-myc induced tumors such as Burkitt's lymphoma.,
Protein name
Branched-chain-amino-acid aminotransferase, cytosolic
Other name
BCAT1; BCT1; ECA39; Branched-chain-amino-acid aminotransferase, cytosolic; BCAT(c); Protein ECA39
Fields
>>Cysteine and methionine metabolism;>>Valine, leucine and isoleucine degradation;>>Valine, leucine and isoleucine biosynthesis;>>Pantothenate and CoA biosynthesis;>>Metabolic pathways;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids;>>Biosynthesis of cofactors
Human protein sequence Database
P54687
Mouse protein sequence database
P24288
Rat protein sequence database
P54690
Cellular localization
Cytoplasm.
Tissue expression
During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
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