Immunogen
Synthesized peptide derived from human UBC9
Specificity
This antibody detects endogenous levels of Human UBC9
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:1000-2000 ELISA 1:5000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.,function:Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Essential for nuclear architecture and chromosome segregation.,pathway:Protein modification; protein sumoylation.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with HIPK1, HIPK2 and PPM1J (By similarity). Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TCF3, TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with human adenovirus E1A and human herpesvirus 6 IE2. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A
Gene Name
UBE2I UBC9 UBCE9
Other name
SUMO-conjugating enzyme UBC9 (EC 6.3.2.-;SUMO-protein ligase;Ubiquitin carrier protein 9;Ubiquitin carrier protein I;Ubiquitin-conjugating enzyme E2 I;Ubiquitin-protein ligase I;p18)
Fields
>>Nucleocytoplasmic transport;>>NF-kappa B signaling pathway;>>Ubiquitin mediated proteolysis;>>MicroRNAs in cancer
Human protein sequence Database
P63279
Mouse protein sequence database
P63280
Rat protein sequence database
P63281
Cellular localization
Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Mainly nuclear (By similarity). In spermatocytes, localizes in synaptonemal complexes (PubMed:8610150). Recruited by BCL11A into the nuclear body (By similarity). .
Tissue expression
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车