Immunogen
The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18. AA range:5-54
Specificity
Phospho-MLC-2 (T17/S18) Polyclonal Antibody detects endogenous levels of MLC-2 protein only when phosphorylated at T17/S18.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ,IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014],
Function
function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.,miscellaneous:This chain binds calcium.,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,
Protein name
Myosin regulatory light chain 12A
Other name
MYL12A; MLCB; MRLC3; RLC; Myosin regulatory light chain 12A; MLC-2B; Myosin RLC; Myosin regulatory light chain 2; nonsarcomeric; Myosin regulatory light chain MRLC3; MYL12B; MRLC2; MYLC2B; Myosin regulatory light chain 12B; MLC-2A; MLC-2; M
Fields
>>Axon guidance;>>Focal adhesion;>>Tight junction;>>Platelet activation;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Shigellosis;>>Salmonella infection
Human gene ID
10398/10627
Human protein sequence Database
P19105/O14950/P24844
Mouse gene ID
67938/98932
Mouse protein sequence database
Rat protein sequence database
P18666/Q64122
Cellular localization
stress fiber,cytosol,brush border,myosin complex,myosin II complex,Z disc,apical part of cell,extracellular exosome,
Tissue expression
Coronary artery,Epithelium,Placenta,Skeletal muscle,Skin,Testis,
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