Molecular weight (DA)
50kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MYLIP. AA range:161-210
Specificity
MYLIP Polyclonal Antibody detects endogenous levels of MYLIP protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008],
Function
developmental stage:Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.,domain:The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.,function:E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Stable or transient overexpression of MIR induces proteasomal degradation of MRLC inhibiting neurite outgrowth in presence of NGF. MIR counteracts the stabilization of MRLC by MIR-interacting saposin-like protein (MSAP/TMEM4) and reduces MSAP-stimulated neurite outgrowth.,pathway:Protein modification; protein ubiquitination.,PTM:Autoubiquitinated.,similarity:Contains 1 FERM domain.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with myosin regulatory light chain (MRLC) and TMEM4.,tissue specificity:Ubiquitously expressed.,
Protein name
E3 ubiquitin-protein ligase MYLIP
Other name
MYLIP; BZF1; IDOL; BM-023; PP5242; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR
Fields
>>Cholesterol metabolism
Human protein sequence Database
Q8WY64
Mouse protein sequence database
Q8BM54
Rat protein sequence database
D3ZDI6
Cellular localization
Cytoplasm . Cell membrane ; Peripheral membrane protein .
Tissue expression
Ubiquitously expressed.
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