Immunogen
The antiserum was produced against synthesized peptide derived from human HSP20 around the phosphorylation site of Ser16. AA range:5-54
Specificity
Phospho-HSP20 (S16) Polyclonal Antibody detects endogenous levels of HSP20 protein only when phosphorylated at S16.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. [provided by RefSeq, Jan 2012],
Function
PTM:The N-terminus is blocked.,similarity:Belongs to the small heat shock protein (HSP20) family.,subunit:May exist as disulfide-linked dimer and monomer.,
Protein name
Heat shock protein beta-6
Other name
HSPB6; Heat shock protein beta-6; HspB6; Heat shock 20 kDa-like protein p20
Human protein sequence Database
O14558
Mouse protein sequence database
Q5EBG6
Rat protein sequence database
P97541
Cellular localization
Cytoplasm . Nucleus . Secreted . Translocates to nuclear foci during heat shock. .
Tissue expression
Muscle,Skeletal muscle,Skin,
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