Molecular weight (DA)
21kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL32. AA range:101-150
Specificity
MRP-L32 Polyclonal Antibody detects endogenous levels of MRP-L32 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008],
Function
similarity:Belongs to the ribosomal protein L32P family.,
Protein name
39S ribosomal protein L32 mitochondrial
Other name
MRPL32; HSPC283; 39S ribosomal protein L32; mitochondrial; L32mt; MRP-L32
Human protein sequence Database
Q9BYag-8
Mouse protein sequence database
Q9DCI9
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Lymph,Trachea,Umbilical cord blood,
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