Molecular weight (DA)
11kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS21. AA range:38-87
Specificity
MRP-S21 Polyclonal Antibody detects endogenous levels of MRP-S21 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice varian
Function
similarity:Belongs to the ribosomal protein S21P family.,subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.,
Protein name
28S ribosomal protein S21 mitochondrial
Other name
MRPS21; RPMS21; MDS016; 28S ribosomal protein S21; mitochondrial; MRP-S21; S21mt
Human protein sequence Database
P82921
Mouse protein sequence database
P58059
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Brain,Cerebellum,Hematopoietic stem cell,Lung,Testis,Uterus,
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