Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS42. AA range:75-124
Specificity
MRP-S32 Polyclonal Antibody detects endogenous levels of MRP-S32 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provid
Function
caution:Has been found in mitochondrial the ribosome large and small subunit. Was erronously (PubMed:11551941) assigned to be MRP-S31.,subunit:Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins. Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.,
Protein name
39S ribosomal protein L42 mitochondrial
Other name
MRPL42; MRPL31; MRPS32; RPML31; HSPC204; PTD007; 39S ribosomal protein L42; mitochondrial; L42mt; MRP-L42; 28S ribosomal protein S32, mitochondrial; MRP-S32; S32mt; 39S ribosomal protein L31, mitochondrial; L31mt; MRP-L31
Human protein sequence Database
Q9Y6G3
Mouse protein sequence database
Q9CPV3
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Brain,Liver,Pituitary tumor,Placenta,Umbilical cord blood,