Immunogen
Synthesized peptide derived from the Internal region of human VPS54.
Specificity
VPS54 Polyclonal Antibody detects endogenous levels of VPS54 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008],
Function
function:May be involved in retrograde transport from early and late endosomes to late Golgi.,similarity:Belongs to the VPS54 family.,subunit:Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS52, VPS53 and VPS54.,
Protein name
Vacuolar protein sorting-associated protein 54
Other name
VPS54; HCC8; Vacuolar protein sorting-associated protein 54; Hepatocellular carcinoma protein 8; Tumor antigen HOM-HCC-8; Tumor antigen SLP-8p
Human protein sequence Database
Q9P1Q0
Mouse protein sequence database
Q5SPW0
Rat protein sequence database
Cellular localization
Golgi apparatus, trans-Golgi network . Membrane . Associates with membranes in an EIPR1-independent manner. .
Tissue expression
Hepatoma,Placenta,Skin,Testis,
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