Molecular weight (DA)
70kD
Immunogen
Synthesized peptide derived from the Internal region of human Prothrombin. AA range 420-470
Specificity
Prothrombin Polyclonal Antibody detects endogenous levels of Prothrombin protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],
Function
catalytic activity:Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.,disease:Defects in F2 are the cause of various forms of dysprothrombinemia [MIM:176930].,disease:Genetic variations in F2 may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.,function:Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protei
Other name
F2; Prothrombin; Coagulation factor II
Fields
>>Phospholipase D signaling pathway;>>Neuroactive ligand-receptor interaction;>>Complement and coagulation cascades;>>Platelet activation;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection;>>Coronavirus disease - COVID-19;>>Pathways in cancer
Human protein sequence Database
P00734
Mouse protein sequence database
P19221
Rat protein sequence database
P18292
Cellular localization
Secreted, extracellular space.
Tissue expression
Expressed by the liver and secreted in plasma.
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