Molecular weight (DA)
42kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human SPOP. AA range:41-90
Specificity
SPOP Polyclonal Antibody detects endogenous levels of SPOP
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],
Function
domain:The MATH domain interacts with H2AFY and BMI1.,function:Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.,miscellaneous:Antigen recognized by serum from scleroderma patient.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the Tdpoz family.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 1 MATH domain.,subunit:Homodimer. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Part of a complex consisting of DAXX, CUL3 and SPOP. Interacts with H2AFY, IPF1/PDX1, BMI1 and DAXX. Interacts with CUL3.,tissue specificity:Widely expressed.,
Other name
Speckle-type POZ protein (HIB homolog 1) (Roadkill homolog 1)
Fields
>>Hedgehog signaling pathway
Human protein sequence Database
O43791
Mouse protein sequence database
Q6ZWS8
Rat protein sequence database
Cellular localization
Nucleus . Nucleus speckle .
Tissue expression
Widely expressed.
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