Molecular weight (DA)
72kD
Immunogen
Synthetic peptide from human protein at AA range: 460-520
Specificity
The antibody detects endogenous PRC1
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012],
Function
function:KIF4A translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Acts as a microtubule-binding and bundling protein both in vivo and vitro. May function as an in vivo cyclin-CDK substrate.,PTM:Phosphorylated; very weak in G1/S phase cells. Much higher levels of phosphorylation are detected at later cell cycle phases, reaching a maximum during mitosis.,similarity:Belongs to the MAP65/ASE1 family.,subcellular location:Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.,subunit:Interacts with the C-terminal Rho
Protein name
Protein regulator of cytokinesis 1
Other name
Protein regulator of cytokinesis 1
Human protein sequence Database
O43663
Mouse protein sequence database
Q99K43
Rat protein sequence database
Cellular localization
Nucleus . Cytoplasm. Cytoplasm, cytoskeleton, spindle pole . Midbody . Chromosome . Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436). Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. Co-localizes with PRC1 in early mitosis and at the spindle midzone from anaphase B to telophase (PubMed:15297875, PubMed:15625105). .
Tissue expression
Overexpressed in bladder cancer cells (PubMed:17409436).
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