Immunogen
Synthesized peptide derived from human APEX2 AA range: 196-246
Specificity
This antibody detects endogenous levels of APEX2 at Human/Mouse
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1：500-2000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012],
Function
catalytic activity:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.,function:May participate in both nuclear and mitochondrial post-replicative base excision repair (BER). In the nucleus functions in the PCNA-dependent BER pathway.,similarity:Belongs to the DNA repair enzymes AP/exoA family.,subcellular location:Colocalized partly with PCNA in nuclear foci.,subunit:Interacts with PCNA. This interaction is increased by misincorporation of uracil in nuclear DNA.,tissue specificity:Highly expressed in cells, adult brain and kidney. Weakly expressed in the fetal brain.,
Gene Name
APEX2 APE2 APEXL2 XTH2
Fields
>>Base excision repair
Human protein sequence Database
Q9UBZ4
Mouse protein sequence database
Q68G58
Rat protein sequence database
Cellular localization
Nucleus. Cytoplasm. Mitochondrion . Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
Tissue expression
Highly expressed in brain and kidney. Weakly expressed in the fetal brain.
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车