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Nogo A Polyclonal Antibody

Polyclonal antibody

Specification

BYab-12774

  • 50UL $180 100UL $255
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Host
Reactiveness
Use
Molecular weight (DA)
220kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Nogo A. AA range:450-499
Specificity
Nogo A Polyclonal Antibody detects endogenous levels of Nogo A protein.
Source
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Background
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
Function
domain:Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.,function:Potent neurite growth inhibitor in vitro and plays a role both in the restriction of axonal regeneration after injury and in structural plasticity in the CNS. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.,online information:Nerve regrowth: nipped by a no-go - Issue 69 of April 2006,online information:The Singapore hu
Gene Name
RTN4
Protein name
Reticulon-4
Abbreviation
Nogo A
Other name
RTN4; KIAA0886; NOGO; My043; SP1507; Reticulon-4; Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5
Fields
>>Alzheimer disease
Human gene ID
57142
Human protein sequence Database
Q9NQC3
Mouse gene ID
68585
Mouse protein sequence database
Q99P72
Rat gene ID
83765
Rat protein sequence database
Q9JK11
Cellular localization
[Isoform A]: Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein ; Cytoplasmic side . Anchored to the membrane of the endoplasmic reticulum (ER) through 2 putative transmembrane domains. Localizes throughout the ER tubular network (PubMed:27619977). Co-localizes with TMEM33 at the ER sheets. .; [Isoform B]: Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein ; Extracellular side . Cell junction . Mainly located on endoplasmic reticulum tubules and sheet edges (PubMed:27786289). Upon ICAM1 engagement, redistributed toward endothelial junctions where interacts with CDH5 (PubMed:21183689). .; [Isoform C]: Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Tissue expression
Isoform A: is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform B: widely expressed except for the liver. Highly expressed in endothelial cells and vascular smooth muscle cells, including blood vessels and mesenteric arteries (PubMed:15034570, PubMed:21183689). Isoform C: is expressed in brain, skeletal muscle and adipocytes. Isoform D is testis-specific.
Storage
-20°C/1 year

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Nogo A Polyclonal Antibody

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