Immunogen
Purified recombinant fragment of human Podocalyxin-like 1 expressed in E. Coli.
Specificity
Podocalyxin-like 1 Monoclonal Antibody detects endogenous levels of Podocalyxin-like 1 protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
Affinity purification
Background
podocalyxin like(PODXL) Homo sapiens This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008],
Function
function:Functions as an antiadhesin that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.,PTM:Glycosylated; contains sialic acid.,similarity:Belongs to the podocalyxin family.,tissue specificity:Glomerular epithelium cell (podocyte).,
Abbreviation
Podocalyxin-like 1
Other name
PODXL; PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1
Fields
>>Salmonella infection
Human protein sequence Database
O00592
Mouse protein sequence database
Q9R0M4
Rat protein sequence database
Cellular localization
Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus . Membrane raft . Membrane ; Single-pass type I membrane protein . In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane o
Tissue expression
Glomerular epithelium cell (podocyte).
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