Immunogen
Purified recombinant human uMtCK protein fragments expressed in E.coli.
Specificity
uMtCK Monoclonal Antibody detects endogenous levels of uMtCK protein.
Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mi
Function
catalytic activity:ATP + creatine = ADP + phosphocreatine.,function:Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.,miscellaneous:Mitochondrial creatine kinase binds cardiolipin.,similarity:Belongs to the ATP:guanido phosphotransferase family.,subunit:Exists as an octamer composed of four MTCK homodimers.,
Protein name
Creatine kinase U-type mitochondrial
Other name
CKMT1A; CKMT; CKMT1B; CKMT; Creatine kinase U-type; mitochondrial; Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK
Fields
>>Arginine and proline metabolism;>>Metabolic pathways
Human gene ID
1159/548596
Human protein sequence Database
P12532
Mouse protein sequence database
P30275
Rat protein sequence database
P25809
Cellular localization
Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Tissue expression
Cerebellum,Lung,PNS,
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车