Molecular weight (DA)
63kD
Immunogen
The antiserum was produced against synthesized peptide derived from human GRK1 around the phosphorylation site of Ser21. AA range:6-55
Specificity
Phospho-GRK 1 (S21) Polyclonal Antibody detects endogenous levels of GRK 1 protein only when phosphorylated at S21.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.,disease:Defects in GRK1 are a cause of congenital stationary night blindness Oguchi type (CSNBO) [MIM:258100]; also known as Oguchi disease. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. CSNBO is an autosomal recessive form associated with fundus discoloration and abnormally slow dark adaptation.,function:Phosphorylates rhodopsin thereby initiating its deactivation.,online information:Retina International's Scientific Newsletter,PTM:Autophosphorylated.,PTM:Farnesylation is required for full activity.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,tissue specificity:R
Protein name
Rhodopsin kinase
Other name
GRK1; RHOK; Rhodopsin kinase; RK; G protein-coupled receptor kinase 1
Fields
>>Chemokine signaling pathway;>>Endocytosis;>>Phototransduction
Human protein sequence Database
Q15835
Mouse protein sequence database
Q9WVL4
Rat protein sequence database
Q63651
Cellular localization
Membrane ; Lipid-anchor . Cell projection, cilium, photoreceptor outer segment . Subcellular location is not affected by light or dark conditions. .
Tissue expression
Retinal-specific. Expressed in rods and cones cells.
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