Immunogen
The antiserum was produced against synthesized peptide derived from human DAPK2 around the phosphorylation site of Ser318. AA range:284-333
Specificity
Phospho-DAPK2 (S318) Polyclonal Antibody detects endogenous levels of DAPK2 protein only when phosphorylated at S318.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-318.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.,tissue specificity:Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.,
Protein name
Death-associated protein kinase 2
Other name
DAPK2; Death-associated protein kinase 2; DAP kinase 2; DAP-kinase-related protein 1; DRP-1
Fields
>>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer
Human protein sequence Database
Q9UIK4
Mouse protein sequence database
Q8VDF3
Rat protein sequence database
Cellular localization
Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
Tissue expression
Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
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