Immunogen
Synthesized peptide derived from human MLK3 (Phospho Thr277+Ser281)
Specificity
This antibody detects endogenous levels of Human,Mouse,Rat MLK3 (Phospho Thr277+Ser281)
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP
Gene Name
MAP3K11 MLK3 PTK1 SPRK
Protein name
MLK3 (Phospho Thr277+Ser281)
Other name
Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25;Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase)
Fields
>>MAPK signaling pathway;>>Non-alcoholic fatty liver disease
Human protein sequence Database
Q16584
Mouse protein sequence database
Q80XI6
Rat protein sequence database
Q66HA1
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.
Tissue expression
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车