Molecular weight (DA)
144kD
Immunogen
The antiserum was produced against synthesized peptide derived from human GAK. AA range:101-150
Specificity
GAK Polyclonal Antibody detects endogenous levels of GAK protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
cyclin G associated kinase(GAK) Homo sapiens In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 C2 tensin-type domain.,similarity:Contains 1 J domain.,similarity:Contains 1 phosphatase tensin-type domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions.,tissue specificity:Ubiquitous. Highest in testis.,
Protein name
Cyclin-G-associated kinase
Other name
GAK; Cyclin-G-associated kinase
Human protein sequence Database
O14976
Mouse protein sequence database
Q99KY4
Rat protein sequence database
Cellular localization
Cytoplasm, perinuclear region . Golgi apparatus, trans-Golgi network . Cell junction, focal adhesion . Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
Tissue expression
Ubiquitous. Highest in testis.
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