Molecular weight (DA)
61kD
Immunogen
The antiserum was produced against synthesized peptide derived from human GK2. AA range:281-330
Specificity
GK2 Polyclonal Antibody detects endogenous levels of GK2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
Function
catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
Protein name
Glycerol kinase 2
Other name
GK2; GKP2; GKTA; Glycerol kinase 2; GK 2; Glycerokinase 2; ATP:glycerol 3-phosphotransferase 2; Glycerol kinase; testis specific 2
Fields
>>Glycerolipid metabolism;>>Metabolic pathways;>>PPAR signaling pathway
Human protein sequence Database
Q14410
Mouse protein sequence database
Q9WU65
Rat protein sequence database
Cellular localization
Mitochondrion outer membrane ; Single-pass type IV membrane protein . Cytoplasm . In sperm the majority of the enzyme is bound to mitochondria. .
Tissue expression
Testis-specific (PubMed:33536340). Expressed in the midpiece of spermatozoa (PubMed:28852571).
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