Molecular weight (DA)
91kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MLTK. AA range:701-750
Specificity
MLTK Polyclonal Antibody detects endogenous levels of MLTK protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165.,function:Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,subcellular location:Tr
Protein name
Mitogen-activated protein kinase kinase kinase MLT
Other name
MLTK; ZAK; HCCS4; Mitogen-activated protein kinase kinase kinase MLT; Human cervical cancer suppressor gene 4 protein; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mixe
Fields
>>MAPK signaling pathway
Human protein sequence Database
Q9NYL2
Mouse protein sequence database
Q9ESL4
Rat protein sequence database
Cellular localization
Cytoplasm . Nucleus . Translocates to the nucleus upon ultraviolet B irradiation. .
Tissue expression
Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
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