Molecular weight (DA)
55kD
Immunogen
The antiserum was produced against synthesized peptide derived from human FRK. AA range:71-120
Specificity
Rak Polyclonal Antibody detects endogenous levels of Rak protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,tissue specificity:Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin.,
Protein name
Tyrosine-protein kinase FRK
Other name
FRK; PTK5; RAK; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5
Human protein sequence Database
P42685
Mouse protein sequence database
Q922K9
Rat protein sequence database
Q62662
Cellular localization
Cytoplasm . Nucleus . Predominantly found in the nucleus, with a small fraction found in the cell periphery.
Tissue expression
Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
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