Immunogen
Synthesized peptide derived from human Calponin-1
Specificity
The antibody can specifically recognize human and mouse calponin-1 protein. In western blotting of Hela, LnCap and mouse muscle lysates, the antibody can label a 34KDa band corresponding to calponin-
Source
Mouse, Monoclonal/IgG2a, kappa
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.146% sodium azide.
Dilution rate
IHC-p 1:100-500, WB 1:200-1000, IF 1:100-500
Purification process (Immunogen)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Function
function:Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.,similarity:Belongs to the calponin family.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 3 calponin-like repeats.,subunit:Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.,tissue specificity:Smooth muscle, and tissues containing significant amounts of smooth muscle.,
Other name
Calponin-1 (Basic calponin;Calponin H1, smooth muscle)
Human protein sequence Database
P51911
Mouse protein sequence database
Rat protein sequence database
Cellular localization
cytoskeleton,focal adhesion,
Tissue expression
Smooth muscle, and tissues containing significant amounts of smooth muscle.
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