Immunogen
Purified recombinant fragment of Nanog (aa20-166) expressed in E. Coli.
Specificity
Nanog Monoclonal Antibody detects endogenous levels of Nanog protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015],
Function
developmental stage:Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.,function:May act as a transcription regulator (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.,function:Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 an
Protein name
Homeobox protein NANOG
Other name
NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog
Fields
>>Signaling pathways regulating pluripotency of stem cells;>>Proteoglycans in cancer
Human protein sequence Database
Q9H9S0
Mouse protein sequence database
Q80Z64
Rat protein sequence database
Cellular localization
Nucleus .
Tissue expression
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
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