Molecular weight (DA)
16kD
Immunogen
The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190
Specificity
TNF-α Polyclonal Antibody detects endogenous levels of TNF-α protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008],
Function
disease:Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition.,disease:Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.,disease:Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated w
Gene Name
TNF TNFA TNFSF2
Protein name
Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular
Other name
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Fields
>>Antifolate resistance;>>MAPK signaling pathway;>>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>NF-kappa B signaling pathway;>>Sphingolipid signaling pathway;>>mTOR signaling pathway;>>Apoptosis;>>Necroptosis;>>TGF-beta signaling pathway;>>Osteoclast differentiation;>>Antigen processing and presentation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>Hematopoietic cell lineage;>>Natural killer cell mediated cytotoxicity;>>IL-17 signaling pathway;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Adipocytokine signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Type I diabetes mellitus;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>P
Human protein sequence Database
P01375
Mouse protein sequence database
P06804
Rat protein sequence database
P16599
Cellular localization
Cell membrane ; Single-pass type II membrane protein .; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted .; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Tissue expression
Blood,Peripheral blood leukocyte,Placenta,Prostatic carcinoma,
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